Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine ruminal epithelium

نویسندگان

  • Kate Keogh
  • Sinead M. Waters
  • Paul Cormican
  • Alan K. Kelly
  • Emma O’Shea
  • David A. Kenny
چکیده

Compensatory growth (CG) is utilised worldwide in beef production systems as a management approach to reduce feed costs. However the underlying biology regulating the expression of CG remains to be fully elucidated. The objective of this study was to examine the effect of dietary restriction and subsequent re-alimentation induced CG on the global gene expression profile of ruminal epithelial papillae. Holstein Friesian bulls (n = 60) were assigned to one of two groups: restricted feed allowance (RES; n = 30) for 125 days (Period 1) followed by ad libitum access to feed for 55 days (Period 2) or (ii) ad libitum access to feed throughout (ADLIB; n = 30). At the end of each period, 15 animals from each treatment were slaughtered and rumen papillae harvested. mRNA was isolated from all papillae samples collected. cDNA libraries were then prepared and sequenced. Resultant reads were subsequently analysed bioinformatically and differentially expressed genes (DEGs) are defined as having a Benjamini-Hochberg P value of <0.05. During re-alimentation in Period 2, RES animals displayed CG, growing at 1.8 times the rate of their ADLIB contemporary animals in Period 2 (P < 0.001). At the end of Period 1, 64 DEGs were identified between RES and ADLIB, with only one DEG identified at the end of Period 2. When analysed within RES treatment (RES, Period 2 v Period 1), 411 DEGs were evident. Genes identified as differentially expressed in response to both dietary restriction and subsequent CG included those involved in processes such as cellular interactions and transport, protein folding and gene expression, as well as immune response. This study provides an insight into the molecular mechanisms underlying the expression of CG in rumen papillae of cattle; however the results suggest that the role of the ruminal epithelium in supporting overall animal CG may have declined by day 55 of re-alimentation.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium

Compensatory growth (CG), an accelerated growth phenomenon which occurs following a period of dietary restriction is utilised worldwide in animal production systems as a management practise to lower feed costs. The objective of this study was to evaluate the contribution of jejunal epithelial to CG in cattle through transcriptional profiling following a period of dietary restriction as well as ...

متن کامل

Effect of Dietary Restriction and Subsequent Re-Alimentation on the Transcriptional Profile of Bovine Skeletal Muscle.

Compensatory growth (CG), an accelerated growth phenomenon which occurs following a period of dietary restriction is exploited worldwide in animal production systems as a method to lower feed costs. However the molecular mechanisms regulated CG expression remain to be elucidated fully. This study aimed to uncover the underlying biology regulating CG in cattle, through an examination of skeletal...

متن کامل

Effect of feed restriction on compensatory growth response of young male goats

Forty eight 8-month-old male goats were divided into three groups. In each group, eight goats were feedrestricted for 45, 60 or 75 days, and eight goats served as the control. Restricted groups were fed with a maintenance ration, and the control goats were fed with a ration supporting 50 g of daily weight gain. At the end of restriction period, the restricted goats were offered the same ration ...

متن کامل

Effects of Fat Supplementation and Chop Length on Milk Composition and Ruminal Fermentation of Cows Fed Diets Containing Alfalfa Silage

The aim of this study was to evaluate the effect of fat supplementation for enhancing milk conjugated linoleic acid (CLA) in early lactation without affecting ruminal fermentation. 24 Holstein dairy cows were assigned to a completely randomized design employing a 2 × 4 factorial arrangement. Factors were particle size (15 and 25 mm) and two different sources of alfalfa supplemented with and wit...

متن کامل

The Effect of 12 Weeks of Aerobic Training with or without Dietary Restriction on the Expression Levels of SIRT3, PGC1-α and SOD2 of Soleusmuscle in Male Rats

Background and Objectives: Background and Objectives: Recently, synergistic effect of dietary restriction (DR) on the exercise induced adaptations has attracted the attention of researchers. The aim of this research was to evaluate the effect of 12 weeks of dietary restriction with or without aerobic training on the gene expression or mRNA of SIRT3, PGC1-α and SOD2 proteins of soleusmuscle tiss...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 12  شماره 

صفحات  -

تاریخ انتشار 2017